PEROXISOMES (MICROBODIES) IN STRIATED MUSCLE CELLS

Microbodies: peroxisomes and glyoxysomes

Microbodies were first reported at the ultrastructural level in the proximal convoluted tubule ofmouse kidney by Rhodin in 1954 (1) and in hepatic parenchymal cells by Rouiller and Bernhard in 1956 (2) at about the time The Journal of Cell Biology was established . They were reported in plants by Porter and Caulfield in 1958 (3) and by Mollenhauer et al. in 1966 (4) . Microbodies are now recogn...

Cytochemical Localization of Catalase in Leaf Microbodies (peroxisomes)

Segments of mature tobacco leaves were fixed in glutaraldehyde, incubated in medium containing 3,3'-diaminobenzidine (DAB) and hydrogen peroxide, and postfixed in osmium tetroxide. Electron microscopic observation of treated tissues revealed pronounced deposition of a highly electron-opaque material in microbodies but not in other organelles. The coarsely granular reaction product is presumably...

Distribution of Peroxisomes (microbodies) in the Nephron of the Rat

The distribution of peroxisomes (microbodies) in the rat nephron was studied cytochemically, using glutaraldehyde- or formaldehyde-fixed tissue, by means of alpha-hydroxy acid oxidase activity in light microscopy or oxidation of 3,3'-diaminobenzidine (DAB) at pH 9 in both light and electron microscopy.The two cytochemical methods show peroxisomes to be nearly sperical particles found only in ce...

Import of proteins into peroxisomes and other microbodies.

Microbodies in Experimentally Altered Cells

A rapid and sustained increase in the number of microbodies in liver and kidney cells can be induced in male rats by ethyl chlorophenoxyisobutyrate (CPIB), a hypolipidemic drug. This phenomenon permits investigation of several aspects of microbody behavior in experimental conditions. Reversal experiments demonstrate that liver cells revert to normal between 2 and 3 weeks after withdrawal of CPI...